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- W2375201594 abstract "Genomic DNA was extracted from leaves of Vitis amurensis,Actinidia arguta,Quercus mongolica,Juglans mandshurica and needles of Pinus sibirica,Pinus pumila by the method of modified CTAB.The isolated genomic DNA length was similar to λDNA(48kb) and is suitable for both PCR amplification and digestion with restriction endonucleases.The absorbance ration(A_(260)/A_(280)) ranged 1.66～1.89.The quality of DNA extracted from third and fourth times suspension was better than from first and second times suspension.The yields of DNA ranged from 15μg·g~(-1) fresh mass(from fourth time suspension J.mandshuric) to 272μg·g~(-1) fresh mass(from third time suspension of V.amurensis).Although from first and second times suspension of P.pumila and P.sibirica wasn't isolated genomic DNA,but from third and fourth times suspension was obtained high quality genomic DNA.The genomic DNA can be used for next analysis via identification of PCR and digestion with restriction endonucleases." @default.
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- W2375201594 date "2006-01-01" @default.
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- W2375201594 title "Extraction of genomic DNA from woody plants and it's identification" @default.
- W2375201594 hasPublicationYear "2006" @default.
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