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- W2375211897 abstract "【Objective】The objectives of the present study were to examine sequence variation in a portion of the mitochondrial NADH dehydrogenase subunit 1 gene (pnad1) within and among Toxocara canis, T. cati, T. malaysiensis, T. vitulorum and Toxascaris leonina, and to study their systematic and population genetics.【Method】 Total genomic DNA was extracted from individuals of Toxocara and Toxascaris species from different geographical locations. The pnad1 of each sample was amplified by a polymerase chain reaction (PCR) using primers end-labelled with γ33P-ATP. Sequence variation in the pnad1 was examined and compared among these nematodes using single-strand conformation polymorphism (SSCP) analysis, followed by DNA sequencing. 【Result】 While the average intraspecific variations in the pnad1 sequences were 0.3%-1.9% for T. canis, 2.8% for T. cati, 0-0.3% for T. malaysiensis from different geographical origins, the interspecific differences were 11.0%-14.1% between T. canis and T. cati, 10.7%-12.4% between T. canis and T. malaysiensis, 12.6%-13.7% between T. canis and T. vitulorum, 17.5%-18.2% between T. canis and T. leonina, 12.0%-13.0% between T. cati and T. malaysiensis, 15.1% between T. cati and T. vitulorum, 18.6%-21.2% between T. cati and T. leonina, 12.4%-12.8% between T. malaysiensis and T. vitulorum, and 18.6%-19.0% between T. malaysiensisand T. leonina. 【Conclusion】The findings of this study showed that the intras-pecific variations in pnad1 within these ascaridoid species were subtle, and significantly lower than interspecific differences, indicating that the pnad1 provides genetic markers for the identification of these ascaridoid nematodes, and should have an important implication for studying systematic, population genetic structures and the molecular ecology of Toxocara and Toxascaris species." @default.
- W2375211897 created "2016-06-24" @default.
- W2375211897 creator A5023968235 @default.
- W2375211897 date "2006-01-01" @default.
- W2375211897 modified "2023-09-25" @default.
- W2375211897 title "DNA Polymorphism in Mitochondrial NADH Dehydrogenase Subunit-1 Within and Among Toxocara and Toxascaris Species" @default.
- W2375211897 hasPublicationYear "2006" @default.
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