Matches in SemOpenAlex for { <https://semopenalex.org/work/W2375212617> ?p ?o ?g. }
Showing items 1 to 59 of
59
with 100 items per page.
- W2375212617 abstract "Objective:To examine the ability of plasmid DNA encoding the human MUC1/Y to elicit antigen-specific CTL responses by gene transfer mediated by dextran coated magnetic iron oxide nanoparticles(DMN)as gene carrier in the magnetic field in vitro.Methods:The dextran coated magnetic iron oxide nanoparticles(DMN)modified with Poly-L-Lysine(PLL)as gene carrier transfect plasmid pEGFP-C1-MUC1/Y as the reporter gene into human dendritic cells(HDC)in the magnetic field using Nd-Fe-B permanent magnet in vitro,and the rate of transfection of plasmid pEGFP-C1-MUC1/Y into human dendritic cells are evaluated under fluorescence microscope and flow cytometer 24 hours later.After transfection,the transfected and nontransfected DC are cocultured with autogenetic T cells respectively,and seven days later with targeted bladder cancer cells(BIU87).The cytotoxic activity or apoptosis of induced CTL to BIU87 is detected with LDH release assay or transmission electron microscope respectively.The IFN-γ secretion of the CTL are detected by ELISA assays.Results:DMN acts as a vector in the magnetic field to transfect reporter gene pEGFP-C1-MUC1/Y into HDC and GFP is successfully expressed,24h after transfection,and the rate of transfection of plasmid pEGFP-C1-MUC1/Y is 15%.The transfected DC(DC-MUC1/Y)can successfully induced CTL with autogenetic T cells cocultured seven days later.The cytotoxic activity of induced CTL to BIU87 by T-DC-MUC1/Y is obviously higher than that by T-DC.Transmission electron microscope map show apoptosis in the earlier period of induced CTL to BIU87,for instance,entoblast disappearance、chromatin enriched around nuclear membrane,et al.There is significant difference in the ability of IFN-γ secretion between the transfected and nontransfected DC groups.Conclusions:Superparamagnetic dextran coated magnetic iron oxide nanoparticles(DMN)as a vector can transfect plasmid pEGFP-C1-MUC1/Y into HDC in the magnetic field successfully,and MUC1/Y protein can enhance the ability of DC to stimulate autogenetic T cells proliferation and induce most potent cytotoxicity of CTL to bladder cancer cells(BIU87)." @default.
- W2375212617 created "2016-06-24" @default.
- W2375212617 creator A5079886307 @default.
- W2375212617 date "2007-01-01" @default.
- W2375212617 modified "2023-09-24" @default.
- W2375212617 title "The induction of specific CTL against bladder cancer by MUC1/Y gene-modified dendritic cells which transfected with dextran coated magnetic iron oxide nanoparticles in the magnetic field" @default.
- W2375212617 hasPublicationYear "2007" @default.
- W2375212617 type Work @default.
- W2375212617 sameAs 2375212617 @default.
- W2375212617 citedByCount "0" @default.
- W2375212617 crossrefType "journal-article" @default.
- W2375212617 hasAuthorship W2375212617A5079886307 @default.
- W2375212617 hasConcept C135983454 @default.
- W2375212617 hasConcept C147969180 @default.
- W2375212617 hasConcept C153911025 @default.
- W2375212617 hasConcept C154317977 @default.
- W2375212617 hasConcept C202751555 @default.
- W2375212617 hasConcept C54009773 @default.
- W2375212617 hasConcept C54355233 @default.
- W2375212617 hasConcept C55493867 @default.
- W2375212617 hasConcept C81885089 @default.
- W2375212617 hasConcept C86803240 @default.
- W2375212617 hasConceptScore W2375212617C135983454 @default.
- W2375212617 hasConceptScore W2375212617C147969180 @default.
- W2375212617 hasConceptScore W2375212617C153911025 @default.
- W2375212617 hasConceptScore W2375212617C154317977 @default.
- W2375212617 hasConceptScore W2375212617C202751555 @default.
- W2375212617 hasConceptScore W2375212617C54009773 @default.
- W2375212617 hasConceptScore W2375212617C54355233 @default.
- W2375212617 hasConceptScore W2375212617C55493867 @default.
- W2375212617 hasConceptScore W2375212617C81885089 @default.
- W2375212617 hasConceptScore W2375212617C86803240 @default.
- W2375212617 hasLocation W23752126171 @default.
- W2375212617 hasOpenAccess W2375212617 @default.
- W2375212617 hasPrimaryLocation W23752126171 @default.
- W2375212617 hasRelatedWork W1991812971 @default.
- W2375212617 hasRelatedWork W1999033202 @default.
- W2375212617 hasRelatedWork W2018029562 @default.
- W2375212617 hasRelatedWork W2038271869 @default.
- W2375212617 hasRelatedWork W2060234319 @default.
- W2375212617 hasRelatedWork W2350212540 @default.
- W2375212617 hasRelatedWork W2354674969 @default.
- W2375212617 hasRelatedWork W2355350862 @default.
- W2375212617 hasRelatedWork W2355662145 @default.
- W2375212617 hasRelatedWork W2370911508 @default.
- W2375212617 hasRelatedWork W2375894169 @default.
- W2375212617 hasRelatedWork W2380171746 @default.
- W2375212617 hasRelatedWork W2383787333 @default.
- W2375212617 hasRelatedWork W2386004655 @default.
- W2375212617 hasRelatedWork W2391475638 @default.
- W2375212617 hasRelatedWork W2398883636 @default.
- W2375212617 hasRelatedWork W2411952037 @default.
- W2375212617 hasRelatedWork W3030126837 @default.
- W2375212617 hasRelatedWork W3032150125 @default.
- W2375212617 hasRelatedWork W3150850570 @default.
- W2375212617 isParatext "false" @default.
- W2375212617 isRetracted "false" @default.
- W2375212617 magId "2375212617" @default.
- W2375212617 workType "article" @default.