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- W2375299737 abstract "Class-specific polyclonal antibodies for O,O-diethyl phosphorothioate pesticides had been generated.A series of generic haptens were synthesized and coupled to bovine serum albumin(BSA) and ovalbumin(OVA) for immunogens and coating antigens,respectively.New Zealand rabbits were immunized with immunogens to obtain corresponding polyclonal antibodies.The effects of hapten structure on antibodies properties and hapten heterology on ELISA sensitivity were also investigated.The results show that haptens with simple spacer-arm can generate antibodies with desired properties,and hapten heterology can improve ELISA sensiti-vity significantly.The polyclonal antibody PAb-H1 shows high sensitivity to seven commonly used O,O-diethyl phosphorothioate pesticides in an indirect competitive ELISA using a heterologous coating antigen(H6-OVA).The 50% inhibition value(IC50) is 0.013 mg/L for coumaphos,0.348 mg/L for parathion,0.022 mg/L for quinalphos,0.035 mg/L for triazophos,0.751 mg/L for phorate,0.850 mg/L for dichlofenthion and 1.301 mg/L for phoxim,respectively.And the limit of detection(LOD) is lower than MRLS of all the seven O,O-diethyl phosphorothioate pesticides in food and agricultural products.Thus,this indirect ELISA with high sensitivity can potentially be used to develop commercial ELISA kits for a sensitive and rapid detection of O,O-diethyl phosphorothioate pesticides residues." @default.
- W2375299737 created "2016-06-24" @default.
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- W2375299737 date "2009-01-01" @default.
- W2375299737 modified "2023-09-25" @default.
- W2375299737 title "Design of Generic Haptens for O,O-Diethyl Phosphorothioate Pesticides and Recognition Properties of Antibodies" @default.
- W2375299737 hasPublicationYear "2009" @default.
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