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- W2375323177 abstract "Objective:To establish an HPLC method for determination of scopolamine,atropine and scopoletin in Huashanshen dripping pills.Methods:The analysis was carried out on a CAPCELL-PAK C18(4.6 mm×250 mm,5 μm)column;The mobile phase was composed of acetonitrile-methanol-disodium hydrogen phosphate buffer solution(15∶4∶81)and the flow rate was 1.0 mL·min-1;The detection wavelengths were set at 210 nm for scopolamine and atropine(0-15 min),and 344 nm for scopoletin(15.1-30 min);The column temperature was 30 ℃.Results:The linear ranges of scopolamine,atropine and scopoletin were 0.04-3.95,0.04-4.10,0.05-0.54 μg(r=0.9999),respectively;The average recoveries(n=6)were 97.58%(RSD=1.3%),98.65%(RSD=1.3%),and 98.42%(RSD=1.8%),respectively.Conclusion:This method is simple,and can be used to determine the above three components with satisfactory accuracy and repeatability." @default.
- W2375323177 created "2016-06-24" @default.
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- W2375323177 date "2012-01-01" @default.
- W2375323177 modified "2023-09-27" @default.
- W2375323177 title "RP-HPLC analysis of scopolamine,atropine andscopoletin in Huashanshen dripping pills" @default.
- W2375323177 hasPublicationYear "2012" @default.
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