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- W2375333463 abstract "Objective To evaluate inhibitory effects of the recombinant plasmid of small hairpin RNA(shRNA)against Ki67 gene,named pSileneer-Ki67,on the growth of human renal carcinoma 786- 0 cells.Methods The pSilencer-Ki67 was transfected into human renal carcinoma cell line 786-0 cells.At 24,48,72,120 and 144 h posttransfection,the effects of pSilencer-Ki67 on the Ki67 expression of human renal carcinoma 786-0 cells were detected by RT-PCR and Western blot.The proliferation of 786-0 cells was measured by MTT assay.The apoptosis of 786-0 cells was tested by TUNEL assay.Results RT- PCR and Western blot analyses revealed that the Ki67 expression levels of 786-0 cells treated with pSi- lencer-Ki67 were reduced significantly.The proliferation of 786-0 cells treated with pSilencer-Ki67 was inhibited and the apoptosis increased.The knock down of Ki67 and inhibition of proliferation and indue- tion of apoptosis mediated by pSileneer-Ki67 were maintained for 144 h and the maximal effect achieved at 48 h or 72 h posttransfection.Conclusion The expression plasmid of shRNA against Ki67 gene can inhibit the proliferation and induce apoptosis for a long period by blocking Ki67 expression of human renal eareinoma cells.The inhibition of Ki67 expression by pSileneer-Ki67 may be a rational approach in gene therapy for renal cancer." @default.
- W2375333463 created "2016-06-24" @default.
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- W2375333463 date "2006-01-01" @default.
- W2375333463 modified "2023-09-23" @default.
- W2375333463 title "Inhibition of human renal carcinoma cell growth by shRNA expression plasmid against Ki67" @default.
- W2375333463 hasPublicationYear "2006" @default.
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