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- W2375366503 abstract "The total internal reflection fluorescence microscopy is one of the most promising bio optical techniques in the world, which has been used to observe single fluorescent molecule directly. This specialized technique specifically illuminates sample with evanescent wave created by total internal reflection, and only a few hundred nanometers depth of sample surface should be excited. As a result, it provides the highest signal to noise ratio and contrast that is unmatched by any other light microscopy technique. Recently, it is gaining popularity with biophysicist to observe single fluorescent molecules. In this paper, the principle of the total internal reflection fluorescence microscopy and the process at home and abroad are systematically reviewed, as well as the application in cell biology is introduced. Future prospect of this technology are also discussed." @default.
- W2375366503 created "2016-06-24" @default.
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- W2375366503 date "2011-08-29" @default.
- W2375366503 modified "2023-09-23" @default.
- W2375366503 title "Total Internal Reflection Fluorescence (Microscopy)" @default.
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- W2375366503 doi "https://doi.org/10.1007/springerreference_67846" @default.
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