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- W2375366995 abstract "InvA gene of Salmonella.spp,Hly gene of Listeria monocytogenes,sa442 gene of Staphylococcus aureus,hlyA gene of Vibrio cholerae and toxR gene of Vibrio parahaemolyticus in the GenBank were analyzed by using the software DNAStar specific gene probes of all the five bacteria labled with biotin were prepared and coupled with fluorescencecoded microspheres.The probes were used for hybridization reaction to PCR products of the five bacteria,and then the liquichip detection technique for detection of all these pathogenic bacteria was established by using liquichip 200 to detect fluorescence signals in the reaction system.The results showed that this method displayed high specificity to PCR products of correspondent bacteria when being used for detection.The sensitivity test indicated that the detecting limitation for the viruses could reach to 50~100 copies/mL.The rapidly high throughput liquichip detection will provide foundation and exploration for further research to detect other pathogenic bacteria and viruses with the same technique." @default.
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- W2375366995 date "2010-01-01" @default.
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- W2375366995 title "Development of a suspension array method for detection of five pathogen." @default.
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