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- W2375389968 abstract "Aim In order to explore and verify the possibility of application of RT-PCR for diagnosis of hemorrhagic fever with renal syndrome(HFRS), and to study the variations of hantaviruses(HV),we synthesized two sets of PCR primers complemented to M gene and S gene of HV respectively and conducted RT-nested PCR for detecting clinical serum samples. The serum samples from 119 HFRS patients (IFA positive) were tested by S gene primers and RT-PCR. The positive rates were 60 5%(1w), 34.1%(1～2w),4%(2～3w),0%(3w) respectively. The serum samples from 31 acute HFRS patients were detected by M gene primers and RT-PCR, only 12 samples were positive (40%).It suggests that the primers complemented to S gene are more efficient than the ones complemented to M gene. Basing on the results of DNA sequencing, it shows that Hantaan virus is a predominant serotype harbored and transmitted in Xi'an district, Shaanxi province. Compared with coding region of S gene of HV strain 76-118, the identity of the nucleotides between them is from 87.2% to 96.0%, identity of the deduced amino acid between them is from 89.0% to 96.0%." @default.
- W2375389968 created "2016-06-24" @default.
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- W2375389968 date "2001-01-01" @default.
- W2375389968 modified "2023-09-24" @default.
- W2375389968 title "HV RNA DETECTION BY REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION AND SEQUENCING OF AMPLIFIED PRODUCTS" @default.
- W2375389968 hasPublicationYear "2001" @default.
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