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- W2375425147 abstract "Objective To establish dual wavelength capillary electrophoresis fingerprints(DW-CEFP) for Shuanghuanglian Jiaonang(SHLJN). Methods The 75 cm×75 μm(the effective length of 57 cm) uncoated fused silica capillary column was used.The triangle and tetrahedron optimization method was employed to select the background electrolyte of 50 mmol·L-1 sodium borate,containing 20 mmol·L-1 β-cyclodextyin and 5% acetonitrile(pH adjusted to 7.55 with phosphoric acid),and the running voltage was 12 kV,in which the detection wavelength was 203 nm and 256 nm.At the same time the chromatographic fingerprint resolution index was applied to optimize the CEFP conditions.The first systematically quantified fingerprint method(FSQFM) was used to evaluate SHLJN quality.The average method was used to integrate different chemical qualitative and quantitative information from the DW-CEFPs. Results The DW-CEFPs were developed,in which 16 and 11 co-possessing peaks were marked under detection wavelength of 203 nm and 256 nm respectively with forsythin peak as the referential peak.The identification results were obtained by the FSQFM which indicated that the quality of S9 was the best,that of S2,S3,S4 and S6 was better,that of S1,S7,S10 and S11 was good,and that of S5,S8 and S12 was acceptable after integration with average method. Conclusion The FSQFM based on DW-CEFP shows good overall qualitative and quantitative identification of SHLJN.The method can serve as a reference to identify and control the quality of SHLJN." @default.
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- W2375425147 date "2012-01-01" @default.
- W2375425147 modified "2023-09-23" @default.
- W2375425147 title "Dual wavelength capillary electrophoresis fingerprints of Shuanghuanglian Jiaonang" @default.
- W2375425147 hasPublicationYear "2012" @default.
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