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- W2375503807 abstract "One pair of primers were designed according to chicken PTH gene sequence published in GenBank.Chicken PTH gene was amplified by RT-PCR from Chicken parathyroideum total RNA extracts.The result showed that the full length of Chicken PTH gene was consisted of 360 bp.The homology of the nucleotides compared to known sequence was 99.6%,since one nucleotide changed from C to A at 159 base。The homology of the nucleotide compared to toad,cattle,horse,people,dog,cat and CEM were 51.4%,50.8%,50.6%,50.3%,50.3%,46.9% respectively.Then,the PTH was sub cloned into the eukaryotic expression vector pCEP4 and such recombinant plasmid was transformed into the host E.coli strain DH5α for identification.The result showed that the recombinant plasmid pCEP4-PTH was constructed correctly,which paved the way of utilizing pCEP4-PTH to regulate calcium metabolism,improve laying performance and research the eggshell quality." @default.
- W2375503807 created "2016-06-24" @default.
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- W2375503807 date "2008-01-01" @default.
- W2375503807 modified "2023-09-26" @default.
- W2375503807 title "Gene Cloning,Sequencing and Eukaryotic Expression Plasmid Construction of Chicken Parathyroid Hormone" @default.
- W2375503807 hasPublicationYear "2008" @default.
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