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- W2375515810 abstract "To inverstigate the effects of progesterone on apoptosis and apoptosis-assoeiated gene expression in splenocytes of menopausal rats, Annexin V FITC/PI flow cytometry assay was used to detect the apoptosis in splenocytes of rats, and RT-PCR was employed to demonstrate the gene expression of Bcl-2 and Bax, Meanwhile, the serum level of progesterone was measured by radio-immunoassay(RIA). Our experimental results showed that the serum level of progesterone in menopausal group of animals was lower than those of the young and progesterone groups, yet there was no difference between these latter two groups. The percentage of the apoptotic cells in splenocytes of progesterone group was similar to that of the menopause group, but the expression of the Bax gene was higher than that of the menopausal rats, These results suggest that the increased ratio of apoptosis in splenocytes of the menopausal rats with aging would be regulated by apoptosis-associated genes, and the treatment with progesterone could not be to reverse the increase of apoptosis in splenocytes of menopausal rats, but could induce the up-regulation of the Bax gene expression." @default.
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- W2375515810 date "2006-01-01" @default.
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- W2375515810 title "Effects of progesterone on apoptosis and apoptosis-associated gene expressions in splenocytes of menopausal rats" @default.
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