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- W2375550849 abstract "Objective To study gene expression influence after the interaction between HCV NS4A and CAML.Methods The vector of pCMV/Myc-NS4A and pcDNA3.1/HisA-CAML were constructed by routine molecular biological methods.The 293 cells were transfected with pCMV/Myc-NS4A and pcDNA3.1/HisA-CAML by lipofectamine,respectively.And pCMV/Myc vector was transfected as negative control Total RNA was isolated and reverse transcribed.The cDNAs were subjected for microarray screening.Results Seven genes were found related to cell apoptosis,among which,4 showed up-regulated and 3 were down-regulated.Conclusions The expression of NS4A protein influenced the expression spectrum of 293-CAML cells,especially some genes related to cell apoptosis pathway.HCV NS4A may have some negative effect on the progress of cell apoptosis,which may lead to chronic HCV infection." @default.
- W2375550849 created "2016-06-24" @default.
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- W2375550849 date "2008-01-01" @default.
- W2375550849 modified "2023-09-25" @default.
- W2375550849 title "Detection of gene expression influence after the interaction between HCV NS4A and CAML with microarray assay" @default.
- W2375550849 hasPublicationYear "2008" @default.
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