Matches in SemOpenAlex for { <https://semopenalex.org/work/W2375554512> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W2375554512 abstract "Objective To investigate the osteogenic potential of adipose tissue-derived stromal cells (ADSCs) exposed to adenovirus containing hBMP-2 cDNA (Adv-hBMP-2) and provide an optimal cell source for gene therapy.Methods ADSCs were obtained from patients who received orthopaedic surgery or liposuction.Human adipose tissue underwent proteolytic digestion to obtain ADSCs.After exposure to Adv-hBMP-2,immunoprecipitation and Western blotting and ELISA were performed for confirming BMP gene transduction.The apoptosis were observed by flow cytometry.ADSCs -Adv-hBMP-2 cells were injected into the hind limb of nude mice.Radiographic and histological evalution were performed on schdule to comfirm the bone formation.Results ADSCs were successfully isolated from human adipose tissue.The transfection efficiency was examined by X-gal staining.Western blotting and ELISA confirmed successful and persistent BMP-2 production by ADSCs -Adv-hBMP-2 cells.The flow cytometry showed there was some negative effect on apoptosis of ADSCs after exposed to adenovirus containing cDNA,but the rate of apoptosis is not so high that compromised the treatment.X-ray and HE sections from hind limb of nude mice subject to injection of Adv-hBMP-2 cells confirmed bone formation at 2nd week.Conclusion Adipose tissue contains abundant ADSCs that can be transfected with BMP-2 gene,with ectopic bone formation.ADSCs may be an ideal source of cells for gene therapy." @default.
- W2375554512 created "2016-06-24" @default.
- W2375554512 creator A5062413297 @default.
- W2375554512 date "2005-01-01" @default.
- W2375554512 modified "2023-09-22" @default.
- W2375554512 title "Ectopic bone formation of hBMP-2 gene transfected human adipose tissue-derived stromal cells" @default.
- W2375554512 hasPublicationYear "2005" @default.
- W2375554512 type Work @default.
- W2375554512 sameAs 2375554512 @default.
- W2375554512 citedByCount "0" @default.
- W2375554512 crossrefType "journal-article" @default.
- W2375554512 hasAuthorship W2375554512A5062413297 @default.
- W2375554512 hasConcept C104317684 @default.
- W2375554512 hasConcept C111599444 @default.
- W2375554512 hasConcept C134018914 @default.
- W2375554512 hasConcept C153911025 @default.
- W2375554512 hasConcept C16930146 @default.
- W2375554512 hasConcept C171089720 @default.
- W2375554512 hasConcept C185592680 @default.
- W2375554512 hasConcept C190283241 @default.
- W2375554512 hasConcept C202751555 @default.
- W2375554512 hasConcept C30352789 @default.
- W2375554512 hasConcept C31507581 @default.
- W2375554512 hasConcept C502942594 @default.
- W2375554512 hasConcept C54009773 @default.
- W2375554512 hasConcept C54355233 @default.
- W2375554512 hasConcept C553184892 @default.
- W2375554512 hasConcept C55493867 @default.
- W2375554512 hasConcept C81885089 @default.
- W2375554512 hasConcept C86803240 @default.
- W2375554512 hasConceptScore W2375554512C104317684 @default.
- W2375554512 hasConceptScore W2375554512C111599444 @default.
- W2375554512 hasConceptScore W2375554512C134018914 @default.
- W2375554512 hasConceptScore W2375554512C153911025 @default.
- W2375554512 hasConceptScore W2375554512C16930146 @default.
- W2375554512 hasConceptScore W2375554512C171089720 @default.
- W2375554512 hasConceptScore W2375554512C185592680 @default.
- W2375554512 hasConceptScore W2375554512C190283241 @default.
- W2375554512 hasConceptScore W2375554512C202751555 @default.
- W2375554512 hasConceptScore W2375554512C30352789 @default.
- W2375554512 hasConceptScore W2375554512C31507581 @default.
- W2375554512 hasConceptScore W2375554512C502942594 @default.
- W2375554512 hasConceptScore W2375554512C54009773 @default.
- W2375554512 hasConceptScore W2375554512C54355233 @default.
- W2375554512 hasConceptScore W2375554512C553184892 @default.
- W2375554512 hasConceptScore W2375554512C55493867 @default.
- W2375554512 hasConceptScore W2375554512C81885089 @default.
- W2375554512 hasConceptScore W2375554512C86803240 @default.
- W2375554512 hasLocation W23755545121 @default.
- W2375554512 hasOpenAccess W2375554512 @default.
- W2375554512 hasPrimaryLocation W23755545121 @default.
- W2375554512 hasRelatedWork W2110004703 @default.
- W2375554512 hasRelatedWork W21838000 @default.
- W2375554512 hasRelatedWork W2320126921 @default.
- W2375554512 hasRelatedWork W2352311679 @default.
- W2375554512 hasRelatedWork W2357078878 @default.
- W2375554512 hasRelatedWork W2364326117 @default.
- W2375554512 hasRelatedWork W2368170626 @default.
- W2375554512 hasRelatedWork W2368315963 @default.
- W2375554512 hasRelatedWork W2373565187 @default.
- W2375554512 hasRelatedWork W2386521873 @default.
- W2375554512 hasRelatedWork W2394174461 @default.
- W2375554512 hasRelatedWork W2403854640 @default.
- W2375554512 hasRelatedWork W2412498415 @default.
- W2375554512 hasRelatedWork W2518332730 @default.
- W2375554512 hasRelatedWork W2614470524 @default.
- W2375554512 hasRelatedWork W2751302063 @default.
- W2375554512 hasRelatedWork W2810490594 @default.
- W2375554512 hasRelatedWork W3028683877 @default.
- W2375554512 hasRelatedWork W3032699913 @default.
- W2375554512 hasRelatedWork W3031450694 @default.
- W2375554512 isParatext "false" @default.
- W2375554512 isRetracted "false" @default.
- W2375554512 magId "2375554512" @default.
- W2375554512 workType "article" @default.