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- W2375581010 abstract "To study the mechanism of Gm SPX3 in response to low phosphorus stress and search for the interaction proteins of Gm SPX3 in soybean,the present research constructed yeast hybrid c DNA library from the soybean root treated with low phosphorus by gateway technology. Furthermore,yeast hybrid c DNA library was screened through the method of yeast two-hybrid using p GBKT7-Gm SPX3 fusion protein as bait. After color reaction of validation,21 positive clones were obtained. Two transcription factors Gm UNE12 and Gm Pos F21 were chosen as the candidate genes for cloning by bioinformatics analysis. Two AD vectors of p GADT7-Gm UNE12 and p GADT7-Gm Pos F21 were constructed. The results of one to one interaction demonstrated that Gm UNE12 and Gm Pos F21 proteins interacted with Gm SPX3 bait protein,respectively." @default.
- W2375581010 created "2016-06-24" @default.
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- W2375581010 date "2016-01-01" @default.
- W2375581010 modified "2023-10-05" @default.
- W2375581010 title "Construction of Yeast Hybrid cDNA Library and Screening of Interaction Proteins of GmSPX3 in Soybean" @default.
- W2375581010 hasPublicationYear "2016" @default.
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