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- W2375602021 abstract "OBJECTIVE To compare the sensitivity and specificity of the three methods-fluorescence quantitative PCR, acid-fast staining of sputum smear, culture with Lowenstein Jensen (L-J) medium and to evaluate the application value of fluorescence quantitative PCR in clinical detection of mycobacterium tuberculosis in sputum smear. METHODS The specimens of sputum were from 240 clinically diagnosed tuberculosis patients and 107 patients with non-tuberculosis respiratory system disease, which were detected by fluorescence quantitative PCR, acid-fast staining of sputum smear, culture with Lowenstein Jensen (L-J) medium, respectively. additionally, using FQ-PCR method detected 14 cases of non-tuberculous mycobacterial DNA to evaluate the specificity of the method of non -tuberculous mycobacteria detection. RESULTS The positive rates and specificity of fluorescence quantitative PCR, acid-fast staining, culture with Lowenstein Jensen (L-J) medium were 52.1% (125 / 240), 24.2% (58 / 240), 35. 4% (85 / 240) respectively. The positive rates by FQ-PCR were higher than those of acid-fast stain and culture with L-J medium. CONCLUSION Fluorescence quantitative PCR is a rapid method for diagnosis tuberculosis, which shows high specificity and sensitivity. It is a useful tool for TB clinic diagnosis." @default.
- W2375602021 created "2016-06-24" @default.
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- W2375602021 date "2013-01-01" @default.
- W2375602021 modified "2023-09-27" @default.
- W2375602021 title "The application of fluorescence quantitative PCR in detection of sputum" @default.
- W2375602021 hasPublicationYear "2013" @default.
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