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- W2375626834 abstract "Stability in simulated gastric fluid(SGF) is regarded as an important parameter in estimation of food allergenicity.The digestive conditions of crustacean allergens(tropomyosin,TM) ,fish allergens(parvalbumin,PV) and nonal-lergenic proteins from muscles of mud crab(Scylla serrata) ,Chinese mitten crab(Eriocheir sinensis) ,grass prawn(Penaeus monodon) ,whiteleg shrimp(Penaeus vannamei) ,Crucian carp(Carassius auratus cuvieri) and Silver carp(Hy-pophthalmichthys molitrix) in SGF were compared.Porcine pepsin was used to simulate digestive proteinases from human.Stabilities of allergens were studied with sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) and Western-blot at different pepsin/protein ratios and pH.It was found that the apparent susceptibility of allergens to prote-olysis was dependent on the ratio of pepsin to allergen.TM was of relatively higher stability;even at pepsin/allergen = 10/1,some stable degradation fragments still existed.Under optimized conditions(0.33U pepsin/μg protein,pH 3.5) ,crustacean allergen TM was relatively resistant to pepsin,but fish allergen PV was rapidly degraded within a short period of time.Further study by Western-blot using sera from crustacean-allergic patients indicated that allergenicity of TM was partially decreased,but the products of TM digested by pepsin still had a little IgE-binding capacity." @default.
- W2375626834 created "2016-06-24" @default.
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- W2375626834 date "2011-01-01" @default.
- W2375626834 modified "2023-09-25" @default.
- W2375626834 title "OPTIMIZATION OF SIMULATED GASTRIC FLUID DIGESTION OF MAJOR ALLERGENS FROM AQUATIC PRODUCTS" @default.
- W2375626834 hasPublicationYear "2011" @default.
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