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- W2375661762 abstract "White spot syndrome virus (WSSV), Infectious hypodermal and hematopoietic necrosis virus (IHHNV), Yellow head virus (YHV) and Taura syndrome virus (TSV) of shrimp are four major viruses which can cause mass mortalities of cultured shrimps. It is an important method to detect these viruses in artermia, the natural food of shrimps, and to prevent the spread of virus diseases. In this study, the technologies of PCR and RT-PCR were developed and applied to detect these viruses in nine samples of artermia. Positive control samples which contained DNA or RNA of WSSV, IHHNV, YHV and TSV yielded evident amplification products showing the ex-pected DNA fragments of 824 bp, 705 bp, 260 bp and 216 bp respectively. But none of these four kinds of viruses can be found from samples of artermia and negative controls. The methods reported here can be used to quarantine WSSV, IHHNV, YHV and TSV in live or frozen shrimps, larva and feed of shrimp in the fields of import and expo" @default.
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- W2375661762 date "2003-01-01" @default.
- W2375661762 modified "2023-09-25" @default.
- W2375661762 title "The detection of four shrimp viruses using PCD and RT-PCR" @default.
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