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- W2375694554 abstract "Time-correlated single photon counting (TCSPC) is based on the detection of single photons of a periodic light signal,the measurement of the detection time of the photons,and the build-up of the photon distribution versus the time in the signal period.The limitations of classic TCSPC were low count rate,long acquisition time,and the fact that the technique was intrinsically one-dimensional.We have developed an advanced TCSPC technique featuring multi-dimensional photon acquisition and a count rate close to the capability of currently available detectors.The technique is able to acquire photon distributions versus wavelength,spatial coordinates,the time on the ps scale,and a second time counting from the start of the experiment.Multi-dimensional TCSPC achieves a near-ideal counting efficiency and a time resolution limited only by the transit-time-spread of the detector.The technique is therefore exceptionally useful for biomedical spectroscopy.Typical applications include time-domain optical tomography,recording of transient phenomena in biological systems,fluorescence lifetime imaging microscopy,FRET experiments in living cells,and the investigation of dye-protein complexes by fluorescence correlation spectroscopy.We demonstrate the potential of the technique for a number of selected applications." @default.
- W2375694554 created "2016-06-24" @default.
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- W2375694554 date "2008-01-01" @default.
- W2375694554 modified "2023-09-25" @default.
- W2375694554 title "Multi-dimensional time-correlated single photon counting:a fast and efficient optical recording technique for biomedical spectroscopy" @default.
- W2375694554 hasPublicationYear "2008" @default.
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