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- W2375694753 abstract "Event-specific qualitative detection of three kinds of genetically modified(GM)maize by multiplex polymerase chain reaction-capillary electrophoresis(CE)with UV detection was established. Three sets of primers were designed to amplify the event-specific fragments of GM maize events(Ly038,Mon863 and Mon810). Multiplex PCR and the separation of CE were systematically optimized,8.0g/L hydroxyethylcellulose in tris-phosphate-EDTA(TBE)buffer as sieving matrix,three event-specific multiplex PCR products were detected by CE in 11.195min.Using Origin program to analyze the CE results and also get the fitting curves of different DNA range,the relative error was between 1.03% and 5.02%. Unpurified and purified normal PCR products were also detected by CE which suggested purification of PCR products was important for CE with UV detection. Compared with normal PCR and agarose gel electrophoresis,the multiplex polymerase chain reaction-capillary electrophoresis method in this study was efficient,rapid,sensitive and low-cost. This event-specific detection method could be widely applied in the fields of food safety and clinical tests." @default.
- W2375694753 created "2016-06-24" @default.
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- W2375694753 date "2011-01-01" @default.
- W2375694753 modified "2023-09-24" @default.
- W2375694753 title "Study on genetically modified maize by multiplex polymerase chain reactioncapillary electrophoresis with UV detection" @default.
- W2375694753 hasPublicationYear "2011" @default.
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