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- W2375706472 abstract "Objective To approach the effect of tumor necrosis factor-like weak inducer of apoptosis(TWEAK) gene on the proliferation of rat bone mesenchymal stem cell(BMSC).Methods The rat BMSCs were isolated and purified from bone marrow of young rats by adherent culture.TWEAK genes were transfected by Ad5CMV-TWEAK,and RT-PCR was used to observe the expression of TWEAK.Then the cells were divided into 3 groups,transfected Ad5CMV-TWEAK group,transfected Ad5CMV-EGFP group and untransfected group,and the MTT assay was used to test the proliferation effect.Results The purified BMSCs were obtained by adherent culture.RT-PCR results showed high TWEAK expression after Ad5CMV-TWEAK transfected.The MTT assay results demonstrated significant difference between Ad5CMV-TWEAK group and untransfected group(P0.05) but no differencebetween the transfected Ad5CMV-EGFP group and untransfected group(P0.05).Conclusion Transfecting TWEAK gene can stimulate BMSCs proliferation,the next research will test if it can promote the differentiation of the cells to show application prospect in bone restore material and implant coating." @default.
- W2375706472 created "2016-06-24" @default.
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- W2375706472 date "2009-01-01" @default.
- W2375706472 modified "2023-09-24" @default.
- W2375706472 title "Studies of tumor necrosis factor-like weak inducer of apoptosis labeling on the proliferation of rat bone marrow mesenchymal stem cells" @default.
- W2375706472 hasPublicationYear "2009" @default.
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