Matches in SemOpenAlex for { <https://semopenalex.org/work/W2375716612> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W2375716612 abstract "Based on the published gene sequence of T7 RNA polymerase(T7 RNAP) in GenBank,a pair of specific primers was designed for amplifying the complete gene of T7 RNAP from E.coli BL21(DE3) cells,the sequencing of the T7 RNA polymerase gene and the eukaryotic expression vector pIRES2-EGFP by Bgl Ⅰ and EcoR Ⅰ restriction enzyme digestions and inserted into the eukaryotic expression vector pIRES2-EGFP to construct the recombinant plasmid pIRES2-EGFP-T7 RNAP.ST cell line stably expressing T7 RNA polymerase was established by using liposome method,G418 selection and cell cloning,at the same time construction of prokaryotic expression vector pET-32a-RED for detection of the red fluorescent protein expression under the control of T7 promoter.The results showed that the ST line of stably expressing T7 RNAP was successfully established.The ST line of stably expressing the T7 RNAP gene could provide a good platform for reverse genetic manipuLation of porcine RNA viruses in vitro." @default.
- W2375716612 created "2016-06-24" @default.
- W2375716612 creator A5028563062 @default.
- W2375716612 date "2012-01-01" @default.
- W2375716612 modified "2023-09-25" @default.
- W2375716612 title "Establishment of a ST cell line stably expressing T_7 RNA polymerase" @default.
- W2375716612 hasPublicationYear "2012" @default.
- W2375716612 type Work @default.
- W2375716612 sameAs 2375716612 @default.
- W2375716612 citedByCount "0" @default.
- W2375716612 crossrefType "journal-article" @default.
- W2375716612 hasAuthorship W2375716612A5028563062 @default.
- W2375716612 hasConcept C104317684 @default.
- W2375716612 hasConcept C121050878 @default.
- W2375716612 hasConcept C150194340 @default.
- W2375716612 hasConcept C153911025 @default.
- W2375716612 hasConcept C199360897 @default.
- W2375716612 hasConcept C22744801 @default.
- W2375716612 hasConcept C2776441376 @default.
- W2375716612 hasConcept C2776449523 @default.
- W2375716612 hasConcept C2780048736 @default.
- W2375716612 hasConcept C33161422 @default.
- W2375716612 hasConcept C40767141 @default.
- W2375716612 hasConcept C41008148 @default.
- W2375716612 hasConcept C54355233 @default.
- W2375716612 hasConcept C547475151 @default.
- W2375716612 hasConcept C67705224 @default.
- W2375716612 hasConcept C82381507 @default.
- W2375716612 hasConcept C86803240 @default.
- W2375716612 hasConceptScore W2375716612C104317684 @default.
- W2375716612 hasConceptScore W2375716612C121050878 @default.
- W2375716612 hasConceptScore W2375716612C150194340 @default.
- W2375716612 hasConceptScore W2375716612C153911025 @default.
- W2375716612 hasConceptScore W2375716612C199360897 @default.
- W2375716612 hasConceptScore W2375716612C22744801 @default.
- W2375716612 hasConceptScore W2375716612C2776441376 @default.
- W2375716612 hasConceptScore W2375716612C2776449523 @default.
- W2375716612 hasConceptScore W2375716612C2780048736 @default.
- W2375716612 hasConceptScore W2375716612C33161422 @default.
- W2375716612 hasConceptScore W2375716612C40767141 @default.
- W2375716612 hasConceptScore W2375716612C41008148 @default.
- W2375716612 hasConceptScore W2375716612C54355233 @default.
- W2375716612 hasConceptScore W2375716612C547475151 @default.
- W2375716612 hasConceptScore W2375716612C67705224 @default.
- W2375716612 hasConceptScore W2375716612C82381507 @default.
- W2375716612 hasConceptScore W2375716612C86803240 @default.
- W2375716612 hasLocation W23757166121 @default.
- W2375716612 hasOpenAccess W2375716612 @default.
- W2375716612 hasPrimaryLocation W23757166121 @default.
- W2375716612 hasRelatedWork W1728715311 @default.
- W2375716612 hasRelatedWork W1972937987 @default.
- W2375716612 hasRelatedWork W2013673762 @default.
- W2375716612 hasRelatedWork W2058659812 @default.
- W2375716612 hasRelatedWork W2091482709 @default.
- W2375716612 hasRelatedWork W2351070197 @default.
- W2375716612 hasRelatedWork W2351830501 @default.
- W2375716612 hasRelatedWork W2363138342 @default.
- W2375716612 hasRelatedWork W2366012763 @default.
- W2375716612 hasRelatedWork W2371820180 @default.
- W2375716612 hasRelatedWork W2371864295 @default.
- W2375716612 hasRelatedWork W2374364667 @default.
- W2375716612 hasRelatedWork W2375301618 @default.
- W2375716612 hasRelatedWork W2375446097 @default.
- W2375716612 hasRelatedWork W2377242346 @default.
- W2375716612 hasRelatedWork W2377819191 @default.
- W2375716612 hasRelatedWork W2379370880 @default.
- W2375716612 hasRelatedWork W2383069129 @default.
- W2375716612 hasRelatedWork W2393400224 @default.
- W2375716612 hasRelatedWork W3036301936 @default.
- W2375716612 isParatext "false" @default.
- W2375716612 isRetracted "false" @default.
- W2375716612 magId "2375716612" @default.
- W2375716612 workType "article" @default.