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- W2375823741 abstract "To prepare monoclonal antibody(MAb) against nucleocapsid protein(NP) of bovine parainfluenza virus type 3(BPIV3),BALB/c mice were immunized with purified recombinant NP(rNP) expressed by E.coli and a hybridoma secreting MAb was screened from fusing the SP2/0 cells with the spleen cells of the immunized BALB/c mice by indirect ELISA coated with BPIV3.The titers of MAb in ascites were 2×106 and 1.28×105 as detected by rNP and BPIV3 coated ELISA,respectively.The MAb was specifically reacted with BPIV3 identified by indirect ELISA,western blot,immunofluroescence assay.The specific tests indicated the MAb had no reaction with infectious bovine rhinotracheitis virus and bovine viral diarrhea virus.The BPIV3 was detected in experimentally infected animals in immunohistochemical test with the MAb.Therefore,this MAb could be used to establish diagnosis method for BPIV3 and further study on the structure and function of NP." @default.
- W2375823741 created "2016-06-24" @default.
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- W2375823741 date "2011-01-01" @default.
- W2375823741 modified "2023-09-22" @default.
- W2375823741 title "Preparation and preliminary application of monoclonal antibody against nucleocapsid protein of bovine parainfluenza virus type 3" @default.
- W2375823741 hasPublicationYear "2011" @default.
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