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- W2375827815 abstract "The present study aimed to examine effects of four penetrating cryoprotectants(dimethyl sulfoxide,glycerol,ethylene glycol and methanol) on fresh and frozen-thawed(freezing temperature was 4℃) sperms of Semilabeo obscurus.The function of fresh and frozen-thawed sperms was evaluated by sperm motility,sperm life and sperm membrane integrity.Results obtained here showed that: four penetrating cryoprotectants presented different negative effects on fresh sperm motility and membrane integrity,methanolethylene glycoldimethyl sulfoxideglycerol;negative effects on fresh sperm life were also observed,methanolethylene glycolglycerol and dimethyl sulfoxide;fresh sperms of S.obscurus [with(36.17±4.06)% for motility,(47.5±3.1)s for life,and(86.22±5.29)% for membrane integrity] were frozen for a short-term;the post-thawing motility of frozen sperms treated with 5% methanol or 5% ethylene glycol were respectively(18.33±1.70)% and(17.83±2.67)%,which were significant different with the fresh sperm motility and higher than those frozen sperms with other treatments;the post-thawing life of frozen sperms treated with 5% methanol or 5% ethylene glycol were respectively(44.3±3.6) s and(42.8±5.5) s,which were longer than those frozen sperms with other treatments;there was no significant difference between post-thawing life of frozen sperms treated with 5% methanol and fresh sperm life;In terms of sperm membrane integrity,5% methanol was the optimal cryoprotectants,which had no significant differences with fresh sperm membrane integrity.These results indicated that: 1) dimethyl sulfoxide and glycerol were not the suitable cryoprotectants for S.obscurus sperm cryopreservation;2) methanol and ethylene glycol had similar effects,which were potential penetrating cryoprotectants for S.obscurus sperm cryopreservation." @default.
- W2375827815 created "2016-06-24" @default.
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- W2375827815 date "2012-01-01" @default.
- W2375827815 modified "2023-09-24" @default.
- W2375827815 title "Effects of Four Penetrating Cryoprotectants on Cryopreservation of Semilabeo obscurus Sperm" @default.
- W2375827815 hasPublicationYear "2012" @default.
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