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- W2375864515 abstract "Based on the established regeneration system,an efficient kanamycin system of clone 3226/3228 of Eucalyptus urophylla×grandis mediated by Agrobacterium was developed.Stem segments were served as explants for transformation,and the factors affecting the transformation frequency,such as pre-culture time,infection time and co-culture time,were discussed by GUS coloration analysis.The concentrations of kanamycin,and antibiotics to inhibit the growth of Agrobacterium,which were suitable to transform Eucalyptus urophylla×Eucalyptus grandis,were discussed.The results indicated that the superior transformation system was as following,infected 3 min directly(OD600=0.5),co-cultured 2 d and then transferred to culture medium with kanamycin 15 mg/L and Tb 300 mg/L for the regeneration of transgenic plantlets.In this experiment,4 and 6 plantlets resistant to kanamycin in clone 3226 and 3228 of Eucalyptus urophylla×grandis respectively,and the conversion rates were 2.3% and 2.6% respectively." @default.
- W2375864515 created "2016-06-24" @default.
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- W2375864515 date "2007-01-01" @default.
- W2375864515 modified "2023-09-28" @default.
- W2375864515 title "Studies on the establish genetic transformat of Eucalyptus urophylla×grandis" @default.
- W2375864515 hasPublicationYear "2007" @default.
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