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- W2375876751 abstract "Objective To explore the practicability of intra,operative bioluminescence ATP,determination assay to evaluate the functional reserve of residual liver parenchyma. Methods Twenty,eight patients who were admitted between Jan. 2005 and Sept. 2005 for hepatic surgery were chosen as research subjects. Hepatic function was assessed before hepatic resection according to Child,Pugh criteria of hepatic functional reserve.Criteria for severity of impaired functional liver parenchyma were set out mainly by histo,pathological change in liver tissues.Clinical features and data of biochemical analysis were also put into account in the process of criteria designing. Liver specimens were delivered by aseptic technique during operation and enzymatically fully digested. Hepatocytes were collected to be used for ATP extraction and bioluminescence ATP determination assay. Results 28 patients underwent hepatic surgery. Total number of patients after severity of impaired liver parenchyma grading were 6 (21.42%), 6(21.42%), 8(28.57%) and 8(28.57%) in normal (N), mild, moderate (mod.) and severe (sev.) and their mean ATP level was 428.49±202.12 mol/cell,782.07±439.19 mol/cell, 874.39±367.93 mol/cell and 1020.764±567.91 mol/cell, respectively. 8 patients (8/8,100%) were suffering from cirrhosis of liver with increased serum total bilirubin and (7/8,87.5%) of patients had liver carcinoma in severe grade. 4 patients (4/8,50%) were suffering from cirrhosis of liver, 2 patients (2/8, 25%) had increased serum bilirubin and 7 patients (7/8,80%) had liver carcinoma including metastasis in mod. grade. Radical surgery with liver transplantation was performed in 6 patients in sev. and 1 patients in mod. group. Conclusion Level of ATP measurement in hepatocyte (HTC) during operation could provide significant information predicting the functional reserve of residual liver parenchyma." @default.
- W2375876751 created "2016-06-24" @default.
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- W2375876751 date "2006-01-01" @default.
- W2375876751 modified "2023-09-25" @default.
- W2375876751 title "Intra,operative assessment of functional reserve of residual liver parenchyma by bioluminescence ATP determination assay" @default.
- W2375876751 hasPublicationYear "2006" @default.
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