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- W2375884069 abstract "Objective To investigate the effects of high-salt medium on the expression of endothelial nitric oxide synthase(eNOS)and related mechanisms in EA.Hy926 cell line.Methods EA.hy926 cells were treated48h with different concentrations of high-salt medium;137 mmol/L was selected as the target concentration intervention in further experiment.The cells were divided into four groups which received intervention for 48 h:DMEM high-glucose medium group(sodium chloride concentration of 109 mmol/L),high-salt medium(sodium chloride concentration of 137 mmol/L),high-salt medium(sodium chloride concentration of 137 mmol/L)+RNA interference,and hypertonic control group(mannitol concentration of 56 mmol/L).High-performance liquid chromatography was used to assay ADMA concentration in cell supernatant;real-time quantitative PCR and Western blot were used to assay the expressions and activities of PRMT-1,RhoA,ROCK and eNOS.Results ADMA concentration,PRMT-1 expression,the mRNA expression of RhoA and ROCK activity increased significantly whereas the protein expression of eNOS significantly reduced in the high-salt group compared with those in the two control groups.High-salt medium + RNA interference restored the above biochemical indicators and upregulated the protein expression of eNOS significantly.Conclusion High-salt medium induces ADMA-mediated RhoA/Rock-1 pathway activation and suppresses eNOS generation." @default.
- W2375884069 created "2016-06-24" @default.
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- W2375884069 date "2013-01-01" @default.
- W2375884069 modified "2023-09-23" @default.
- W2375884069 title "The effects and mechanism of high-salt medium on eNOS expression in human umbilical vein endothelial cell line" @default.
- W2375884069 hasPublicationYear "2013" @default.
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