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- W2375929650 abstract "In this study,optimization for ISSR amplification system Trichosanthes kirilowii Maxim.was determined in two factors(DNA template and primer) at three levels respectively,combined with single factor experiment and orthogonal designed.A suitable ISSR reaction system was established,namely 20 μL reaction system containing 0.5 μmol/L primer,10 μL of 2 × Master Mix,35 ng of DNA template.The optimal extended time and annealing temperature for ISSR-PCR reaction were proposed by gradient PCR.And the best ISSR PCR procedures included predenaturing at 94 ℃ for 5 min;30 cycles of denaturing at 94 ℃ for 30s,annealing at 55 ℃ for 1 min and extension at 72 ℃ for 45 s.The amplification was completed by holding the reaction mixture at 72 ℃ for 7 min and holding at 4 ℃.The annealing temperature was different according to different primers." @default.
- W2375929650 created "2016-06-24" @default.
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- W2375929650 date "2013-01-01" @default.
- W2375929650 modified "2023-09-26" @default.
- W2375929650 title "Study on Optimization for ISSR Reaction System in Trichosanthes kirilowii Maxim" @default.
- W2375929650 hasPublicationYear "2013" @default.
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