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- W2375969730 abstract "To observe the humoral and cellular immune responses in BALB/c mice induced by the nucleic acid vaccine with the full-length elp gene of Echinococcus multilocularis.30 BALB/c mice were divided into 3 groups.and inⅠ,Ⅱ and NS gooups of mice,each mouse was intramuscularly injected with 100μg of plasmid pcDNA3.1,100μg of the eukaryotic expression recombinant plasmid pcD-ELP with the full-length elp gene of Echinococcus multilocularis or equal volume of normal saline respectively.Each mouse was injected three times at a two-week interval.Before and at different times after immunization,specific antibodies of IgG1,IgG2a and IgG2b were detected with ELISA.IFN-γ,IL-4 and IL-12 produced by peripheral mononuclear cell(PMNC) of spleen defore and after stimulation of E.multilocularis antigen wereexamined with Double-Antibody-Sandwich ELISA kits.The effects of the nucleic acid vaccine on the proliferation of spleen lymphocytes were evaluated by a ~(3)H-TdR method.In Ⅱ groups of mice,specific IgG1,IgG2a and IgG2b antibodies were detectable in four weeks after the first dose immunization,then gradually increased and reached highest OD value at the end of experiment,i.e.at the end of the seventh week after first immunization.The amount of IFN-γ produced by spleen PMNC of Ⅰand Ⅱ group reached 50 μg/ml and 55μg/ml in their culture supernatants respectively.After specific antigen stimulation,the amount of IFN-γ of Ⅰ and Ⅱ group were 44pg/ml and 101.5pg/ml respectively.However,INF-γ in NS group, IL-4 and IL-12 in all 3 groups were below the threshold of the kits.Compared with that of NS group,the proliferations of spleen lymphocytes of both Ⅰ and Ⅱ group were enhanced.Their CPM values were 85 and 123 times as high as that of NS group.The capacities of lymphocyte proliferation were even higher after in vitro stimulation of specific antigen or ConA.It is evident from the above observations that the nucleic acid vaccine with the full-length elp gene of E.multilocularis is able to induce both cellular and humoral immune responses in BALB/c mice." @default.
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- W2375969730 date "2006-01-01" @default.
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- W2375969730 title "Immune response in mice induced by nucleic acid vaccine wih elp gene of Echinococcus multilocularis" @default.
- W2375969730 hasPublicationYear "2006" @default.
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