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- W2375978797 abstract "Objective: Genes encoding five sequential pathway steps of taxol biosythesis, which are geranylgera-nyl diphosphate synthase(GGPPS), taxadiene synthase(TS), taxadiene-5α-hydroxylase(THY5α), taxadiene-5α-O-acetyl-transferase(TAT) and taxane-10β-hydroxylase(TDH), were installed in a single E.coli host. Then throughbiosynthesis, the host can produce an intermediate taxadiene. Methods: Brick gene assembly method is a flexiblestandard for biological part assembly, through it we mutated the vector pET30 a, designing BglⅡ/BamHⅠ and XbaⅠ/SpeⅠ expression cassette. Results: The BglⅡ/BamHⅠ and XbaⅠ/SpeⅠ multi-gene expression cassette was de-signed successfully. The five genes were achieved single genes expressin individually and multi-genes expression si-multaneously, GGPPS and TS, THY5α, TAT and TDH. Conclusion: The use of BglBrick method can help fulfillthe rapid assembly of. taxol biosynthesis enzymes and sequential expression." @default.
- W2375978797 created "2016-06-24" @default.
- W2375978797 creator A5041659230 @default.
- W2375978797 date "2014-01-01" @default.
- W2375978797 modified "2023-09-24" @default.
- W2375978797 title "Expression of Taxol Biosynthetic Enzymes via Bio-BglBrick Method" @default.
- W2375978797 hasPublicationYear "2014" @default.
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