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- W2375995505 abstract "Objective To analyze changes in the mumber of Bacteroides,Fusobacterium and Clostridium in colorectal cancer patients by real-time fluorescence quantitative PCR and reveal the role and significance of gut microflora in colorectal cancer-associated molecular pathogenesis.Method The feces of preoperative colorectal cancer patients(n=40) and healthy volunteers(n=40) were collected.Specif primers were designed according to the target gene sequences of Bacteroides,Fusobacterium and Clostridium.Bacterial genome DNA extracted from fecal samples were quantified by SYBR Green I real-time fluorescence quantitative PCR to analyze bacterial amounts.Result In normal control group and study group the levels of Bacteroides(8.76±0.77;9.85±0.88),Fusobacterium(7.94±1.25;10.0±1.65) and Clostridium(3.54±0.70;6.56±0.68),B.fragilis(2.12±0.48;4.07±1.77),F.necrosis(2.31±0.26;7.62±2.68) and C.botulinum(2.76±1.16;5.43±1.21) were significantly higher than those of healthy controls(P0.05).Conclusion The fecal Bacteroides,Fusobacterium and Clostridium composition in colorectal cancer patients are significantly higher than those of healthy controls,which suggests that the colorectal cancer is related to intestinal microflora." @default.
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- W2375995505 date "2010-01-01" @default.
- W2375995505 modified "2023-09-26" @default.
- W2375995505 title "Real-time fluorescence quantitative-PCR analysis of the variation of Bacteroides, Fusobacterium and Clostridium in patients with colorectal cancer." @default.
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