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- W2376021523 abstract "Objective To study the effect of liquid nitrogen cryopreservation on percentages of CD4+T and CD8+ T lymphocyte,and to explore the feasibility of cryopreserved PBMCs as a quality control panel for lymphocyte immonophenotypes.Methods Whole blood specimens from healthy adults were collected and PBMCs were isolated using standard ficoll-gradient centrifugation.After stored in liquid nitrogen,the PBMC samples were thawed at different intervals and then were assessed for percentages of CD4+ T and CD8+T lymphocyte,viability and recovery.Besides,the effects of thawing methods,lymphocyte immonophenotypes reagents,fixed time on percentages of CD4+ T and CD8+ T lymphocyte were evaluated.Results The percentage of CD4+ T and CD8+ T lymphocyte of PBMCs cryopreserved in liquid nitrogen from 0 to 12 monthes and from 12 to 24 monthes remained stable.The average CV of CD4+T and CD8+T percentages of PBMC cryopre-served at different intervals between 0 to 12 monthes were 14.5% and 9.8% respectively,with viabilities of 85.4%～ 94.3% and recoveries of 33.3%～97.8%.The average CV of CD4+T and CD8+T percentages of PBMCs cryopreserved at different intervals between 12 to 24 monthes were 7.1% and 6.5% respectively,with viabilities of 88.3%~98.5% and recoveries of 52.3%～101%.The results of using different thawing methods and lymphocyte immonophenotypes reagents remained stable.The average CV of CD4+T and CD8+T percentages were 8.1% and 6.4%.The thawed PBMCs could be detected correctly within 48 hours after fixed in polyformaldehyde.Conclusion Cryopreserved PBMCs could be used as a potential panel for quality control of lymphocyte immonophenotypes." @default.
- W2376021523 created "2016-06-24" @default.
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- W2376021523 date "2008-01-01" @default.
- W2376021523 modified "2023-09-23" @default.
- W2376021523 title "Preliminary Study on a Potential Panel Used for Quality Control of Lymphocyte Immonophenotypes" @default.
- W2376021523 hasPublicationYear "2008" @default.
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