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- W2376066869 abstract "Objective To investigate the effects of FHIT gene on the malignant growth of lung cancer cells with different gene background and its mechanism of cancer inhibition. Methods A mammalian expression vector PEGFP-FHIT was transfected into the lung cancer lines by lipofectamine.We tested the transfected cancer cell lines by RT-PCR,immunochemical stain and Western blot.The inhibition growth efficacy of extraneous FHIT was evaluated by clonogenic survival assay and flow cytometry.The expression of p21~(Waf/cip) protein was examined by Western blot.Results All of the transfected cancer cell lines had the expression of FHIT gene and protein.The clonal formation rate of 801D-FHIT(46.3%),A2-FHIT(43.7%),A549-FHIT(32.7%) was lower than that of 801D(58.2%),A2(60.3%),A549(52.8%)(P0.01).Cell cycle analysis by FACS showed that 801D-FHIT,A2FHIT and A549-FHIT cells were blocked at G_1,S and G_2 phase,respectively.A significant increase in p21~(Waf/cip) protein expression was noticed in FHIT expression clones 801D-FHIT,A2-FHIT and A549-FHIT compared with that of the control parental cell lines.Conclusion Extrogenous FHIT gene can suppress the proliferation in different FHIT gene status and regulate the cell cycle.There is a different cell cycle arrest in three sorts of lung cancer cell lines with different expression of FHIT gene.Our data suggests that observed growth-inhibitory effect in FHIT-reexpressioning cells could be related to cell cycle arrest and linked to the increased p21~(Waf/cip) protein expression." @default.
- W2376066869 created "2016-06-24" @default.
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- W2376066869 date "2005-01-01" @default.
- W2376066869 modified "2023-09-23" @default.
- W2376066869 title "EXOGENOUS FHIT GENE INHIBITS THE GROWTH OF HUMAN LUNG CANCER CELL LINES WITH DIFFERENT FHIT STATUS" @default.
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