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- W2376078617 abstract "In order to establish a rapid assay to detect all genotypes of porcine bocavirus(PBoV),a set of primers were designed to amplify PBoV1/PBoV2 and PBoV3/PBoV4/PBoV5,respectively,according to the published sequences of all the genotypes of PBoV in GenBank.By optimizing the annealing temperature and the proportion of the two sets of primers,a duplex PCR method was established to simultaneously detect PBoV1/PBoV2 and PBoV3/PBoV4/PBoV5.18 clinical samples collected from pig herds with diarrheain Sichuan Province were detected by the established PCR assay. In result,the assay was specific,sensitive and reproducible. In addition,the infection of PBoV was existed in Sichuan Province,and ten of eighteen samples were PBoV positive,including seven for PBoV3/PBoV4/PBoV5,two for PBoV1/PBoV2,and one for the coinfection of PBoV1/PBoV2 and PBoV3/PBoV4/PBoV5.The results showed that the established duplex PCR assay successfully detected all the genotypes of PBoV,and could distinguish between PBoV1/PBoV2 and PBoV3/PBoV4/PBoV5. The duplex PCR provides a basis for the diagnosis and prevention of PBoV in Sichuan Province." @default.
- W2376078617 created "2016-06-24" @default.
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- W2376078617 date "2013-01-01" @default.
- W2376078617 modified "2023-09-23" @default.
- W2376078617 title "Development and application of the duplex PCR for detection of porcine bocavirus" @default.
- W2376078617 hasPublicationYear "2013" @default.
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