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- W2376095026 abstract "Objective To explore factors affecting the in vitro culture of Helicobacter pylori.Methods Colonies and the bacterial morphology of H.pylori were observed at different times.Bacterial nucleic acids were stained with fluorescent SYTO9/PI to ascertain appropriate culturing times and estimate survival time after exposure to air.Results The bacterial colonies were as small as the tip of a needle and the bacteria had a typical spiral/corkscrew morphology.After culturing for 3 days,the colonies enlarged to 1 mm in size and the bacteria had a spherical morphology.Fluorescence staining indicated the H.pylori were dead after exposure to air for 4 hours.Conclusion The optimal culturing time for H.pylori is 3 days,and the resulting bacteria will have a typical morphology.Helicobacter pylori survives for less than 4 hours when exposed to air." @default.
- W2376095026 created "2016-06-24" @default.
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- W2376095026 date "2013-01-01" @default.
- W2376095026 modified "2023-09-23" @default.
- W2376095026 title "Study of factors affecting the in vitro culture of Helicobacter pylori" @default.
- W2376095026 hasPublicationYear "2013" @default.
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