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- W2376140010 abstract "To improve the selection efficiency of restoring gene for BT-type cytoplasmic male sterility(CMS) in japonica rice,we designed a functional marker InDel-Rf1a based on a 574 bp insertion/deletion(InDel) in Rf1a locus between japonica restorer line and CMS line reported by previous studies and used it to identify the genotypes of Rf1a locus in 72 indica or japonica rice materials.The results showed that there was no 574 bp deletion in indica conventional rice varieties,restorer and maintain lines and they could restore the fertility of BT-type CMS in japonica rice,with genotypes of Rf1aRf1a;However,the same deletion was detected in most of japonica conventional rice varieties,except in Aichi 106 and Yijing 12,and they had the ability to maintain sterility of BT-type CMS lines,with genotypes of Rf1aRf1a.For further verification of the detection effect with this marker for different genotypes in this locus,we used it to amplify the DNA of different japonica rice restorer lines,CMS lines,hybrid F1 and the F2 population derived from a cross between 863A and Ninghui 8,and three genotypes in Rf1a locus could be distinguished distinctly by gel electrophoresis." @default.
- W2376140010 created "2016-06-24" @default.
- W2376140010 creator A5076313660 @default.
- W2376140010 date "2013-01-01" @default.
- W2376140010 modified "2023-09-24" @default.
- W2376140010 title "Development and Application of a Functional Marker Associated with Fertility-restoring Gene for BT-type Cytoplasmic Male Sterility(CMS) in japonica Rice" @default.
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