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- W2376152880 abstract "Objective To establish the quantifying technology for gene expression change(GEC) of GADD45 after X-ray irradiation,and to discuss the feasibility of the GEC of gene GADD45 as a radiation biodosimeter.Methods Healthy whole-blood model was used to separate lymphocytes after irradiation,total mRNA was extracted and reverse transcripted to get cDNA,SYBR Green real-time PCR technology was used to assay gene GADD45 expression at mRNA level exposed to different doses.Then housekeeping gene GAPDH was chosen and applied to rectify the quantity of every dose.Results The up-regulation of gene GADD45 was dose-dependent in transcription level after irradiation.It reached the peak at 4 Gy irradiaton and still stayed in relatively high level at 8 Gy.A linear regression equation was established as =3.408+1.528x,R2=0.787.After its being exposed to 2 Gy X-ray,mRNA expression showed up-regulated at 1 hour,reached the peak at the 4th hour,but still not returned to the beginning level.Conclusion GEC of GADD45 analysis using SYBR Green real-time PCR is rapid,sensitive and reproducible." @default.
- W2376152880 created "2016-06-24" @default.
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- W2376152880 date "2008-01-01" @default.
- W2376152880 modified "2023-09-26" @default.
- W2376152880 title "Real-time PCR detection of the change of GADD45 gene expression in irradiated lymphocytes" @default.
- W2376152880 hasPublicationYear "2008" @default.
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