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- W2376185771 abstract "In this study,Ovalbumin(OVA) was used as a protein carrier to couple with 3-nitrotyrosine by glutaraldehyde method.The artificial antigen for 3-nitrotyrosine was thus prepared as immunoantigen in enzyme-linked immunosorbent assay(ELISA) protocol;Ultraviolet spectroph-otometry and sodium dodecyl-sulfatepolycrylamide gel electrophonesis(SDS-PAGE) were applied to determine it,and the concentration of the conjugate was determined by BCA method and the conjugated ratio was estimated.The concentration of the conjugate was 1.69 mg/mL;the molecular weight was more than 43KD by SDS-PAGE.Based on the UV spectrum,the excursion of the conjugate was different from 3NT and OVA,that is to say,the conjugate was succeed and the coupling rate was about 5.4,the high titer anti-3NT pAb was been produced in serum of immunized BALB/C mice.The results showed that the artificial antigen for 3-nitrotyrosine was synthesized successfully,which made it possible to establish method of 3NT residues in diseases." @default.
- W2376185771 created "2016-06-24" @default.
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- W2376185771 date "2013-01-01" @default.
- W2376185771 modified "2023-09-28" @default.
- W2376185771 title "Synthesis and Identification of the Antigens for the 3-nitrotyrosine" @default.
- W2376185771 hasPublicationYear "2013" @default.
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