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- W2376198120 abstract "Objective To investigate a approach of constructing tissue-engineered skin substitute consisting of epidermal keratinocytes seeded on a fibroblast-containing collagen matrix layer.Methods Collagen Ⅰ extracted from rat-tail tendons was used to construct scaffold-collagen-sponge and collagen-gel onto which the fibroblasts were seeded during logarithmic growth phase and cultured for one week,then 4.0×10~5cells/cm~2 of keratinocytes were seeded on the surface of the collagen-scaffold and cultured for another 3 days,then the skin graft substitutes were elevated to air-liquid interface to allow the maturation of the epidermis cultured for 6 weeks.All specimens underwent the gross,HE staining,histological,immuno-histochemistric and electron microscope evaluation.Results The cells we harvested were pure human fibroblasts and keratinocytes could be used as seeding cells for the construction of tissue-engineered artificial skin.The epidermal histological appearance and the constitution of composite skin was found to be similar to that of normal skin.The autologous skin substitute was constructed successfully with positive collagen Ⅳ,fibronectin and laminin staining. Conclusion This method supplies tissue engineered skin with enough seeding cells simply and suitably,the constitution of the composite skin was similar to human being's skin." @default.
- W2376198120 created "2016-06-24" @default.
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- W2376198120 date "2005-01-01" @default.
- W2376198120 modified "2023-09-25" @default.
- W2376198120 title "Experimental Study of the Reconstruction of Tissue-Engineered Skin by Collagen-Sponge" @default.
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