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- W2376202357 abstract "Aim To study the effect of hydrogen sulfide(H2S) on pulmonary apoptosis in the lipopolysaccharide(LPS)-induced acute lung injury(ALI) in rats.Methods Fourty-eight ♂ SD rats were randomly divided into six groups(n=8): Control group,LPS group,LPS+NaHS Low-dose group,LPS+NaHS Middle-dose group,LPS+NaHS High-dose group and LPS+PPG group.Model of ALI was reproduced by injection of LPS(5 mg·kg-1) via sublingual vein in LPS group,LPS+NaHS Low,Middle,High dose groups and LPS+PPG group.Different doses of sodium hydrosulfide(NaHS) or DL-propargylglycine(PPG) were administered at 3 h after administering LPS in LPS+NaHS Low,Middle,High dose groups and LPS+PPG group respectively,while normal saline was administered in control group and LPS group.The rats were sacrificed at 6 hours after normal saline or LPS injection via sublingual vein,and the lung tissue was obtained.The apoptosis rate of pulmonary cells was evaluated by flow cytometry(FCM).Meanwhile,the expressions of Bcl-2 and Bax were respectively detected by immunohistochemisty(IHC),and the expressions of Caspase-3 and Caspase-9 were analysed by Western blot.Results Compared with those of control group,apoptosis rate of pulmonary cells was markably increased,Caspase-3,Caspase-9 and Bax protein expressions were markably increased,and Bcl-2 protein expression and Bcl-2/Bax were significantly decreased in LPS group(P0.05 or P0.01).Compared with those of LPS group,apoptosis rate of pulmonary cells was significantly decreased in LPS+NaHS Middle and High dose groups,Bcl-2 protein expression was significantly increased and Caspase-3,9 protein expressions were significantly decreased in LPS+NaHS High dose group,Bax protein expression was significantly decreased in LPS+NaHS Middle and High dose groups,Bcl-2/Bax was significantly increased in LPS+NaHS Low,Middle and High dose groups(P0.05 or P0.01).Compared with those of model group,apoptosis rate of pulmonary cells,Bcl-2 protein expression and Bcl-2/Bax were significantly increased,while Bax and Caspase-3,9 protein expressions were markably decreased in LPS+PPG group(P0.05 or P0.01).Conclusion Pulmonary cells apoptosis is increased in early ALI induced by LPS.H2S can decrease pulmonary cells apoptosis through down-regulating Bax and Caspase-3,9 protein expressions,and up-regulating Bcl-2 protein expression." @default.
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- W2376202357 date "2012-01-01" @default.
- W2376202357 modified "2023-09-23" @default.
- W2376202357 title "Effects of hydrogen sulfide on pulmonary apoptosis induced by lipopolysaccharide in rats" @default.
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