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- W2376207802 abstract "Objective The growth of cultured osteoblasts o n the surface of natural coral(NC)was investigated to determine if NC w as suitable for cell seeding scaffold i n the regeneration of tissue enginee red bone.Method NC was treated by chemical and physic al procedure,microstructure of treated NC was observed by scanning electronic microscope(SEC);Rabbit calvaria osteoblasts were c ultured and seeded onto NC at the density of 5×106 /ml in 10μl medium.Cell adhesion,spreading a nd growth on the surface of NC were observed by phase contrast microscope and SEC.ALP activity of O B growing on the surface of NC was also measured.Result NC has inter -connective three dimen-sional pores and the porosity was about 36%.1day after seeding,OBs could adhere and spread well on the surface of NC.5days after seeding,OBs covered the surface of NC and maintained their ALP activity.Conclusion NC has good compatibility to OBs and h as the character of porosity,which suggested that NC was suitable for OBs seedi ng scaffold in the regeneration of tissue engineering bone." @default.
- W2376207802 created "2016-06-24" @default.
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- W2376207802 date "2001-01-01" @default.
- W2376207802 modified "2023-09-25" @default.
- W2376207802 title "Observation of osteoblasts growth on porous natural coral" @default.
- W2376207802 hasPublicationYear "2001" @default.
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