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- W2376221245 abstract "CVNH(cyanovirin-N homology,CVNH) is the homology protein of CVN,which is a novel anti-HIV glycoprotein.In the previous study,we have firstly reported the full-length sequences of CVNH gene cloned from Ceratopteris thalictroides,and constructed prokaryotic expression plasmid pET32a-CtCVNH.The plasmid DNA was transformed into Rosetta 2(DE3) E.coli cells for expression of the recombinant protein.In the study,we optimized culture conditions of CVNH protein expression,including the types and ingredients of the culture medium,initial pH,A600nm value,induction agents and concentrations,induction time,and expression time,respectively.The optimized culture conditions contained TB medium with 24 g · L-1 yeast extract and 72 g · L-1 tryptone,initial pH 8.0,and lactose induced for 6 hours at A600nm 0.6,which would increase CVNH productivity to 1.9 times than before.This result laid a foundation to explore the drug development of CNVN." @default.
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- W2376221245 date "2013-03-25" @default.
- W2376221245 modified "2023-09-23" @default.
- W2376221245 title "Optimization for Culture Condition of CVNH in Escherichia coli, A Novel Anti-HIV(Human Immunodeficiency Virus) Protein" @default.
- W2376221245 hasPublicationYear "2013" @default.
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