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- W2376298081 abstract "In order to obtain a recombined linoleic acid isomerase with catalytic activity,the in vitro directed evolution was applied on linoleic acid isomerase gene of Lactobacillus acidophilus strain AS1.1854.After two consecutive errorprone PCR amplification and a DNA reorganization,mutant gene fragments were cloned into the pET30a plasmid vector and transformed into E.coli so as to form a mutation library of the isomerase.A recombinant bacteria strain capable of expressing soluble recombinant enzyme protein was screened out after IPTG-induced expression.The result of UV spectrophotometer showed that the activity of the recombinant enzyme protein was 8.2 U." @default.
- W2376298081 created "2016-06-24" @default.
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- W2376298081 date "2010-01-01" @default.
- W2376298081 modified "2023-09-27" @default.
- W2376298081 title "RESEARCH ON DIRECTED EVOLUTION OF LINOLEIC ACID ISOMERASE GENE OF LACTOBACILLUS ACIDOPHILUS" @default.
- W2376298081 hasPublicationYear "2010" @default.
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