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- W2376312786 abstract "A voltammetric α-fetoprotein immunosensor was constructed by using an electropolymerized neutral red (PNR) film on a glassy carbon electrode. A high density of amino groups of PNR was used to covalently attach protein probes via the aid of glutaraldehyde cross-linking,leading to significantly improved detection stability compared with conventional entrapment methods. HRP was used instead of bovine serum albumin to block unspecific sites,and the amperometric response signal was amplified,thus the sensitivity of the immunosensor was enhanced. Under the optimized conditions,the immunosensor showed a specific response to AFP in the range from 1.00 to 10.0 ng/mL and 10.0 to 200 ng/mL with a detection limit of 0.40 ng/mL. The fabrication steps are simple and convenient and the immunosensor has high stability and sensitivity." @default.
- W2376312786 created "2016-06-24" @default.
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- W2376312786 date "2010-01-01" @default.
- W2376312786 modified "2023-09-23" @default.
- W2376312786 title "A voltammetric α-fetoprotein immunosensor based on electropolymerized neutral red film" @default.
- W2376312786 hasPublicationYear "2010" @default.
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