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- W2376355817 abstract "The method of liquid nitrogen grinding and ultrasonic were applied to crush Nostocflagelliforme colonies and cells.The crudes were isolated by differential centrifugation;thylakoid membranes were purified by high speed centrifugation with gradient density sucrose;the thylakoid membrane proteins were extracted and separated by SDS-PAGE.The results suggested that:(1)Thylakoid membrane of colonies on dehydration for 6h(water contenting 51.2%)and on dehydration for 24h(water contenting 14.9%)were purified by many tims differential centrifugation and then by high speed centrifugation with gradient density sucrose.(2)14 bands of thylakoid membrane protein were separated by SDS-PAGE,their mass weight mainly ranged from 25kD to 60kD.8 kinds of protein were identified by MALDI-TOF-TOF/MS and database searching.According to their biological functions,these thylakoid membrane proteins were divided into 4 categories,including photosynthesis proteins(photosystem Ⅱ manganese-stabilizing protein PsbO,F1F0ATP synthase subunit alpha,F1F0ATP synthase subunit beta),binding domain protein(hemerythrin HHE cation binding domain-containing protein),carbohydrate-selective porin(carbohydrate-selective porin OprB),unknown proteins(hypothetical protein Npun_R1321,Npun_R3785 and N9414_02186).They might play an important role in photosynthesis of N.flagelliforme." @default.
- W2376355817 created "2016-06-24" @default.
- W2376355817 creator A5014166956 @default.
- W2376355817 date "2014-01-01" @default.
- W2376355817 modified "2023-09-24" @default.
- W2376355817 title "Separation and Identification of Thylakoid Membrane Proteins in Nostoc flagelliforme on Dehydration" @default.
- W2376355817 hasPublicationYear "2014" @default.
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