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- W2376372227 abstract "Objective:Obtain the entire gene Secoisolariciresinol Dehydrogenase(SDH)and constructed it into the IPTG inducible prokaryotic expression plasmid (PGEX-6P-1) in E.coli. Method: Obtaining the entire SDH gene fragment by PCR splicing, then amplify the entire SDH gene sequence by PCR, insert it into the prokaryotic express vector PGEX-6p-1.Then the recombinant plasmid was transferred into E.coli BL21 and induced with IPTG. Finally, the results was detected by SDS-PAGE. Result: The SDH gene was amplified and the recombinant plasmid was constructed successfully. The expressed protein induced by 1.5mmol/L IPTG was the same size as expectant protein Secoisolariciresinol Dehydrogenase.And the best inducing time is 4h with 37℃,250r/min.Conclusion: Successfully construct the recombinant plasmid, and the recombinant Secoisolariciresinol Dehydrogenase protein was well expressed in E.coli by inducing with IPTG." @default.
- W2376372227 created "2016-06-24" @default.
- W2376372227 creator A5048354020 @default.
- W2376372227 date "2009-01-01" @default.
- W2376372227 modified "2023-09-28" @default.
- W2376372227 title "The Entire Gene SDH Splicing and Prokaryotic Expression" @default.
- W2376372227 hasPublicationYear "2009" @default.
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