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- W2376374788 abstract "Objective: To investigate the role of oxidative DNA damage in development of human lung cancer and its molecular mechanism of carcinogenesis. Methods A specific monoclonal antibody to the biomarker of oxidative DNA damage,8-hydroxydeoxyguanine (8 - OH - dG) , and biotin-streptavidin immuno-staining were used to detect oxidative DNA damage in 150 cases of human lung cancer tissues, 120 adjacent lung tissues without cancer cells,40 benign lung lesions and 40 normal lung tissues. Protein expressions of P53 , C-MYC, K-RAS, BCL - 2 and hTERT (human telomerase reverse transcriptase)in lung tissues were assessed by immuno-histochemistry. The relationship between the oxidative DNA damage and these genes was analyzed. We also detected the oxidative DNA damage during the process of carcinogenesis in human bronchial epithelial cells induced by Benzo (a) pyrene diol-epoxide (BPDE) and sulfide nickel (NiS) in vitro. Result The positive rate of oxidative DNA damage in lung cancer specimens,adjacent lung tissues,benign lung lesions and normal lung tissues was 92. 7 % (139 of 150) , 17. 5% (21 of 120) , 10. 0% (4 of 40) and 5. 0% (2 of 40) respectively. The level of oxidative DNA damage in lung cancer tissues was significantly higher than that of adjacent lung tissues, benign lung lesions and normal lungs(P 0.01). With the lung cancer patients stratified by sex, age, cell types and smoking history, these characteristics were not correlated with the level of oxidative DNA damage. In the investigation of the relationship between the oxidative DNA damage and five cancer related genes, higher oxidative DNA damage levels were observed in lung cancer patients with over-expression of K-RAS and BCL-2,whereas the expressions of P53,C-MYC and hTERT were not correlated with the level of oxidative DNA damage. During the process of malignant transformation and carcinogenesis of human bronchial epithelial cells induced by BPDE and NiS in vitro, oxidative DNA damage emerged before cell transformation, and quantity of that was consistent with the progress of cell cancerization. Conclusion Oxidative DNA damage may play an important role in the initiation of human lung cancer. And K-RAS and BCL - 2 genes may correlate with oxidative DNA damage in the process of lung carcinogenesis." @default.
- W2376374788 created "2016-06-24" @default.
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- W2376374788 date "2003-01-01" @default.
- W2376374788 modified "2023-09-25" @default.
- W2376374788 title "A Study on the Role of Oxidative DNA Damage in Development of Human Lung Cancer" @default.
- W2376374788 hasPublicationYear "2003" @default.
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