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- W2376384729 abstract "Objectiv To construct human intestinal trefoil factor(hITF) by recombinant technique and to study the effect and potential mechanism of hITF in protection of intestinal epithelial ceils.Method The recombinant hITF plasmid was constructed with a yeast-based expression vector pPIC9 in Pichia pas- toris,and then purified from the yeast fermentation broth by anion-and cation-exchange chromatography. The parified protein was analysed by electrospray mass spectrometry.The effects of recombinant hITF (10~(-9)10~(-5)mol/L) on enterocyte (IEC-6) morphology,proliferation and migration were examined. Results The recombinant hITF was successfully constructed,and verified by Moldi-TOF-TOF analysis. The recombinant hITF promoted cell migration in a concentration range of 10~(-9) to 10~(-5) mol/L,but had no effet on cell morphology and proliferation.Conclusion The recombinant hITF protects intestinal mucosa by promoting cell migration." @default.
- W2376384729 created "2016-06-24" @default.
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- W2376384729 date "2007-01-01" @default.
- W2376384729 modified "2023-09-22" @default.
- W2376384729 title "Effects of recombinant human intestinal trefoil factor in protection of intestinal epithelial cells" @default.
- W2376384729 hasPublicationYear "2007" @default.
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