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- W2376403345 abstract "The establishment of marine mollusks permanent cell lines is one of the scientific problems that has not been conquered. The main reasons are lacking appropriate culture medium and cell factors. In this study,we investigate the effects of vertebrate cell factors on primary cell culture and biomineralization ability of mantle cells from the pearl oyster Pinctada fucata. Mantle tissues of 3-year-old pearl oysters were selected to culture the primary cells. Epidermal growth factor( EGF),endothelial cell growth supplement( ECGS),insulin-like growth factor-1( IGF-1) and basic fibroblast growth factor( b FGF) were added separately into the culture medium. The effects were evaluated by comparing the cell viability,adherence capacity,migration ability and expression levels of four matrix protein genes( pif80,n16,msi7 and accb p).According to the results,the cell viability,adherence and migration ability were significantly improved by EGF. The expression levels of pif80,n16 and accbp all increased under the stimulation of this factor. After ECGS treatment,the adherence and migration abilities of primary cells were improved,and the expression levels of pif80,n16 and msi7 were increased significantly. The cell viability,adherence,migration ability as well as the expression levels of msi7 were increased by IGF-1 treatment. However the expression level of pif80,n16 and accbp decreased after cells were treated by this factor. After b FGF treatment,the cell viability and adherence capacity were improved. The expression levels of pif80,n16 and accbp were also increased by this factor. These results suggested that vertebrate cell factors may be used as cell culture supplements,which were able to increase cell viability and biomineralization ability of mantle cells in Pinctada fucata. This study therefore may provide some theoretical references for the establishment of marine mollusks permanent cell lines." @default.
- W2376403345 created "2016-06-24" @default.
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- W2376403345 date "2015-01-01" @default.
- W2376403345 modified "2023-09-24" @default.
- W2376403345 title "Effects of vertebrate cell factors on cell culture and biomineralization ability of mantle cells from Pinctada fucata" @default.
- W2376403345 hasPublicationYear "2015" @default.
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