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- W2376424141 abstract "The effect of addition of Tween-80 on aggregation of cIFN expressed by Pichia pastoris in 5 L fermentor was investigated,and the cell growth,cIFN level,and bioactivity of cIFN were analyzed and compared.The electrophoresis results revealed that the aggregation of cIFN was alleviated,and the content of cIFN monomer was increased remarkably from 41.2% to 82.3% when Tween-80 was added at the dosage(ratio of mass to volume) of 0.01% at induction phase temperature 25℃.Bioactivity of cIFN in the supernatant of fermentation was elevated to 3.73×108IU·mL-1 from 1.91×108 IU·mL-1 when Tween-80 was added at dosage of 0.04%.The cIFN monomer content could be improved markedly after 0.02% Tween-80 was added.And the effects of addition of Tween-80 on total protein and biomass were negligible." @default.
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- W2376424141 date "2008-01-01" @default.
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- W2376424141 title "Study on the Effect of Tween-80 on Aggregation of Consensus Interferon-α Expressed by Pichia Pastoris" @default.
- W2376424141 hasPublicationYear "2008" @default.
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